MULTI-COLOR-PAM-II
About the system
Modulated measurements can be used to probe many processes related to electron transport and regulation. Flash-based measurements allow the monitoring of P680 redox kinetics, period-4 oscillations and Car-triplet induction and decay kinetics. By combining both techniques in a single measurement, users can precisely probe the relative QA redox state during continuous light measurements. With two detectors and free choice of detection wavelengths, it is also possible to probe variable PS I fluorescence. When working with cyanobacteria or diatoms, for example, the availability of five excitation wavelengths enables excitation of the outer antennae rather than only the chlorophylls of the core antennae. Both the flash lamp, which can be used stand-alone, and the two detectors with multi-colour emitter can be used for measurements on leaves, seagrasses or macroalgae.New features
The new elements of the MULTI-COLOR-PAM-II add whole new domains to the measurement portfolio of the instrument, making it even more a multi-function fluorometer.- High time resolution: The new STK-detectors provide a time resolution of 0.3 µs.
- Time-resolved flash responses: As the first commercially available instrument of its kind, the MULTI-COLOR-PAM-II lets researchers measure the fluorescence yield during a saturating µs flash and differentiate carotenoid triplet quenching (TQ) from donor-side dependent quenching (DQ).
- Highly precise: The flash responses feature extremely precise and reproducible timing to ensure detailed analysis. The flash shape approaches a rectangle, as the LEDs require only about 0.5 µs to reach full intensity. The software also includes a special routine for flash-profile correction.
- Extremely intense flashes: The EDST emitter-detector unit achieves flash intensities exceeding 1,000,000 µmol photons m-2 s-1 (440 nm), yielding more than 1 excitation per µs.
- Pump-probe: Double flash experiments with variable dark intervals ∆t (from 1 µs to 10 ms) allow highly flexible relaxation measurements of various forms of quenching.
- Period-4 oscillations: You can generate flash trains at frequencies up to 100 Hz (10 ms time intervals) to probe the S-states of the oxygen-evolving complex.
- Leaf measurements: Although we originally designed the instrument for suspension measurements, researchers can use the EDST emitter-detector (STK-flashlamp) unit independently to measure leaves. Additionally, an upcoming configuration places two emitters at a 45° angle relative to the multi-color emitter, empowering you to detect two wavelengths of leaf fluorescence simultaneously.
- STK embedded in PAM recording: Fast switching (8-10 µs) lets you easily alternate between fast non-modulated and PAM measurements. You can place a single turnover (ST) flash anywhere along a traditional recording—such as an O-I1-I2-P or Slow Kinetics recording—so the resulting STK reveals exact details on the state of PS II at the precise moment of the flash. Altogether, these features make the MULTI-COLOR-PAM-II a highly versatile fluorometer, allowing you to probe and monitor PS II and the photosynthetic electron transport chain in numerous ways.
Included in the package?
- Power-and-control-Unit MCP-II-C
- Multi-color Emitter Head MCP-II-E
- Multi-Color Emitter Head MCP-II-E
- Multi-Color Detector Head MCP-II-D1
- Emitter Head for fast Fluorescence Measurements MCP-II-EDST
- Combined Detector Head for Double Channel and Fast Fluorescence MCP-II-D2ST
- Power Supply MINI-PAM/L
- System Control and Data Acquisition
- Saturation Pulse Analysis
- Stand with Base Plate ST-101
- Transport Box
On this Page
Email: sales@ictinternational.com.au
Phone: 02 6772 6770 (between 9am to 5pm New South Wales time)
Schedule a meeting with our expertsSpecifications
| Parts | Specifications |
| Power-and-Control-Unit | ARM microcontroller (480 MHz), Fast Kinetics up to 128,000 points with 14 bit resolution, ST Kinetics up to 15,000 points with 14 bit resolution, unlimited storage for Slow KineticsUSB 2.0, USB 3 compatible
Windows computer with PamWin-4 software |
| Multi-Color Emitter Head MCP-II-E | Chip-on-board multi-wavelength measuring light LED emitter: 400, 440, 480, 540, 590, 625 nm for pulse-modulated measuring light; 20 intensity settings and 14 pulse frequency settings
Chip-on-board multi-wavelength actinic LED array: 440, 480, 540, 590, 625 and 420-640 nm (white) for continuous actinic illumination, max. 5000 µmol photons m-2 s-1; saturating single turnover flashes, max. 200000 µmol photons m-2 s-1; multiple turnover pulses, max. 12000 µmol m-2 s-1 PAR, adjustable between 1 and 800 ms Far-Red LED: peak wavelength 730 nm |
| Multi-Color Detector Head MCP-II-D1 | Signal detection: PIN photodiode with special pulse preamplifier for measuring fluorescence changes with maximum time resolution of 10 µs
Filter Box: for up to 14 mm filter thickness Standard detector filter: long-pass filter >650 nm (3 mm RG 665) plus short-pass filter SP 710 |
| Emitter Head for fast Fluorescence Measurements MCP-II-EDST | High quality 450 nm ST-flashes of variable duration and intensity up to 1 mol photons m-2 s-1; Actinic light (450 nm); Far Red light (740 nm); STK-detector for surface detection of direct fluorescence with sub-µs time resolution |
| Combined Detector Head for Double Channel and Fast Fluorescence MCP-II-D2ST | STK & PAM-Detector (switchable)
includes: PAM-detector equivalent to MCP-II-D1 and STK-detector for non-modulated fluorescence with sub-µs time resolution Filter box: for up to 14 mm filter thickness Standard detector filter: long-pass filter >650 nm (3 mm RG 665) plus short-pass filter SP 710 |
| Saturation Pulse Analysis | Measured: Ft, F0, FM, F, F0‘ (also calculated), FM‘. Fast polyphasic rise and decay kinetics (time resolution up to 10 μs). PAR using Spherical Micro Quantum Sensor US-SQS/WB or Mini Quantum Sensor US-MQS/WB
Calculated: F0‘ (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate), C/F0 (constant fraction of F0 not constituting PS II chlorophyll fluorescence) Measured: Calculated: F0’ (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate), C/F0 (constant fraction of F0 not constituting PS II chlorophyll fluorescence) |
Accessories
- Optical Unit for Suspensions ED-101US/MD
- Temperature Control Block ED-101US/T
- Spherical Micro Quantum Sensor US-SQS/WB
Support Documents
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Case Studies and Application Notes
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