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MONITORING-PAM and MICRO-PAM

Fluorometer for Long Term Monitoring of Photosynthesis
The MONITORING-PAM and MICRO-PAM enable long-term, non-invasive monitoring of primary photosynthetic productivity. It allows users to connect multiple measuring heads simultaneously to a single data acquisition unit or interface box, and the system operates reliably under harsh environmental conditions.
MICRO-PAM with MONI-DA

About the System

MONITORING-PAM

The MONITORING-PAM system supports up to four cylindrical, rugged, and weather-resistant MONI-HEAD/485 units. Each unit measures PAM fluorescence, ambient photosynthetically active radiation (PAR), and temperature, while the interface box connects all units to a PC. When paired with the MONI-DA stand-alone data acquisition system, the setup supports and operates up to seven measuring heads simultaneously.

System Configuration

  • MONI-SET: Requires a PC or laptop and supports up to four measuring heads
  • MONI-SET-DA: Operates with or without a PC and supports up to seven measuring heads
Both configurations offer aquatic and terrestrial versions. Users can also add a WiFi or satellite modem for remote monitoring, which transmits data to a laptop or PC with a slight delay.

Areas of Application

  • Terrestrial and marine ecophysiological research
  • Continuous monitoring and long-term measurement of primary photosynthetic production
  • Detection of sustained non-photochemical energy quenching during long-term stress periods

Included in the Package

Aquatic Version
  • Titanium Measuring Head (MONI-HEAD/S) – available in sets of 3 or 4
  • PC Interface Box (MONI-IB4/LANS)
  • MONI-DA Data Acquisition Unit (included with MONI-SET-DA only)
  • Transport box
Terrestrial Version
  • Aluminium Measuring Head (MONI-HEAD/S) – available in sets of 3 or 4
  • PC Interface Box (MONI-IB4/LANS)
  • MONI-DA Data Acquisition Unit (included with MONI-SET-DA only)
  • Transport box

MICRO-PAM

The MICRO-PAM provides a compact and lightweight fluorometer for long-term monitoring of primary photosynthetic productivity in smaller samples such as plants, lichens, and corals. Like the MONITORING-PAM, each measuring head includes sensors that measure PAM fluorescence, ambient PAR, and temperature. The system is available in sets of three or four measuring heads by default. Special adapter boxes (MICRO-HUB) allow users to connect up to 16 measuring heads to a single MONI-DA unit.

Amber and Blue Versions

Users can choose configurations with or without a stand-alone data acquisition unit, similar to the MONITORING-PAM. While the MONITORING-PAM offers terrestrial and aquatic versions, the MICRO-PAM provides either blue light (MICRO-3B-SET3 or SET4) or amber light (MICRO-3A-SET3 or SET4) versions. Systems that use blue light perform reliably in both terrestrial and aquatic research. However, cyanobacteria absorb amber light more efficiently than blue light. As a result, amber light produces a stronger PAM signal. For this reason, MICRO-PAM systems equipped with MICRO-HEAD/3A measuring heads work particularly well for examining cyanobacterial mats, lichens, and crusts that contain cyanobacteria.

Included in the Package

  • PC Interface Box (MONI-IB4/LAN for online configuration; MONI-IB1 for stand-alone configuration)
  • Measuring Heads – set of 3 or 4 (amber or blue version)
  • MONI-DA Data Acquisition Unit (for stand-alone configuration only)
  • Transport box

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Any questions?

Email: sales@ictinternational.com.au

Phone: 02 6772 6770 (between 9am to 5pm New South Wales time)

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Specifications

MONITORING-PAM

Parameters Terrestrial Aquatic
Measuring Head
Housing Water-tight aluminum cylinder with one end featuring a ball lens for focusing modulated measuring light, actinic light and saturating light pulses on the sample, and collecting fluorescence. Opposite end of tube: M12 5-pole socket for RS485/power line As described for MONI-HEAD/485 but polyoxymethylene (POM) replaces aluminum as housing material and a special underwater 6-pole socket replaces the standard 5-pole RS485 socket. Waterproof down to a depth of 75 m
Sample Clip Consisting of 2 aluminum frames (3.5 x 2.5 cm), held together by a special O-ring, and mounted at a distance of 2.5 cm from the MONI-HEAD/485 optical window. Angle between optical axis of the MONI-HEAD/485 and sample clip plane: 120° As described for MONI-HEAD/485 but titanium replaces aluminum
Cables Data/power cable, 10 m standard length (between MONI-HEAD/485 and MONI-IB4/LAN, MONI-HEAD/485 and MONI-DA, or MONI-DA and MONI-IB1) Special underwater cables for communication and power. Standard length: 10 m (MONI-HEAD/S to MONI-IB4/LANS, MONI-HEAD/S to MONI-DA/S, or MONI-DA/S to MONI-IB1/S)
Power consumption Peak loads during saturating pulses 7 W. During measuring mode 0.1 W
Operating Temperature 15 to +40 °C
Light Emission
Modulated fluorescence excitation Blue power LED (typical peak wavelength 470 nm, full width at half maximum 22 nm). Photosynthetically active radiation (PAR) of measuring light at level of the sample clip range from 0.15 to 1.5 μmol m-2 s-1 at low modulation frequencies (5 to 25 Hz), and from 1.5 to 22.5 μmol m-2 s-1 at high modulation frequency (100 Hz)
Actinic light Same power LED as for modulated light. At level of sample clip, maximum photosynthetically active radiation of actinic light and saturating flashes of 1500 and more than 8500 μmol m-2 s-1
Sensors
Fluorescence PIN-photodiode protected by longpass filter (50% transmittance at 645 nm). Selective window amplifier to measure pulse amplitude modulated (PAM) fluorescence
Photosynthetically active radiation (PAR) Integrated quantum sensor (photodiode protected by near infrared filters) measuring the radiation reflected by a 1.3 x 0.7 cm area of an optically diffuse Teflon sheet, 1 mm thick, mounted at the edge of the leaf clip
Temperature Integrated-circuit temperature sensor on circuit board

 

MICRO-PAM

Parameters Blue Amber
Measuring Head
Housing Polymer housing with optical block and fiber connector on one side, and a combined power line/RS485 socket on the opposite side
Sample Clip Consisting of 2 aluminum frames (3.5 x 2.5 cm), held together by magnet, 0.4 cm distance between sample plane of leaf clip and lightguide, angle between sample plane and optical light guide: 60°
Cables RS 485 data/power cable, 10 m standard length, connecting MICRO-HEAD/3B and MICRO IB4 PC Interface Box. RS 485 adapter cable, 0.6 m long, to connect MICRO-HEAD/3B to optional PC Interface Box MONI IB4/LAN or to optional MONI DA system for data acquisition
Power consumption Peak loads during saturating pulses 3 W. During measuring mode 0.15 W
Operating Temperature 15 to +40 °C
Light Emission
Modulated fluorescence excitation Blue power LED (typical peak wavelength 460 nm, full width at half maximum 22 nm). Photosynthetically active radiation (PAR) of measuring light at level of the sample clip range from 0.15 to 1.5 μmol m-2 s-1 at low modulation frequencies (5 to 25 Hz), and from 1.5 to 22.5 μmol m-2 s-1 at high modulation frequencies (100 Hz) Amber power LED (typical peak wavelength 598 nm, full width at half maximum 27 nm). Photosynthetically active radiation (PAR) of measuring light at level of the sample clip range from 0.15 to 1.5 μmol m-2 s-1 at low modulation frequencies (5 to 25 Hz), and from 1.5 to 22.5 μmol m-2 s-1 at high modulation frequencies (100 Hz)
Actinic light Same power LED as for modulated light. At sample level of leaf clip, 3000 μmol m-2 s-1 maximum PAR of actinic light, 8000 μmol m-2 s-1 maximum PAR of saturating flashes
Sensors
Fluorescence PIN-photodiode protected by longpass filter (50% transmittance at 645 nm). Selective window amplifier to measure pulse amplitude modulated (PAM) fluorescence
Photosynthetically active radiation (PAR) External LS-C sensor for selective PAR measurement, range 0 to 7000 μmol m-2 s-1, cosine-corrected for light incident at angles between -30° to +30° from the surface normal
Temperature Thermocouple: Ni-CrNi, wire diameter 0.1 mm, -20 to +60 °C

 

Accessories (optional)

  • MICRO-HEAD/SG
  • MICRO-HUB
  • MONI-DA/WIFI
  • MONI-DA/SWIFI

Support Documents

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Case Studies and Application Notes

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