DUAL-PAM-100
P700 & Chlorophyll Fluorescence Measuring System
The DUAL-PAM-100 is an optimised and high-performance measuring device for simultaneous assessment of P700 and Chlorophyll Fluorescence. Optional emitter-detector modules can be added for measuring key photosynthesis parameters such as delta-pH, membrane potential, NADPH.
About the system
The DUAL-PAM measuring system is available as MODULAR Version (DUAL-PAM-100) and as FIBER Version (DUAL-PAM/F). The MODULAR Version has light sources and signal detection located in separate measuring heads where the samples are placed in between, and it uses the transmission mode to measure photosystem I absorption changes. Meanwhile, the FIBER Version has light sources and signal detection in the central unit where the sample is in front of the fiberoptics. Further, it uses the remission mode to measure photosystem I absorption changes. The measuring heads of the MODULAR version can be easily exchanged. Using various accessory modules, the MODULAR VERSION can measure the electronic band shift caused by membrane energization, NADPH fluorescence, fluorescence of pH-sensitive dyes, and fluorescence at two different wavebands. The FIBER version lacks this flexibility but may be advantageous when only photosystem II and photosystem I activities are of interest.Included in the package
| Modular version | Fiber version |
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Any questions?
Email: sales@ictinternational.com.au
Phone: 02 6772 6770 (between 9am to 5pm New South Wales time)
Schedule a meeting with our experts
Specifications
| Modular | Fiber | |
| Power-and-Control-Unit | ||
| General Design | 2 x AVR-RISC (8 MHz) + 4 MB SRAM; 256 000 data points with 12 bit resolution can be stored | Microcontroller: 2 x AVR-RISC (8 MHz) + 4 MB SRAM; 256 000 data points with 12 bit resolution can be stored |
| Measuring Light | Using DUAL-E: P700 dual-wavelength emitter. Sample wavelength 830 nm, reference wavelength 875 nm
Using DUAL-DB/DR: 460 nm (DUAL-DB) or 620 nm (DUAL-DR) |
P700-dual-wavelength-emitter: Sample wavelength 830 nm, reference wavelength 870 nm. Fluorescence emitter: 620 nm |
| Actinic red light | Using DUAL-E: Far-red LED lamp: 730 nm. Chip-on-board (COB) LED array: 635 nm for continuous actinic illumination, maximum 3000 μmol m-2 s-1 PAR. Saturating single turnover flashes, maximal 200 000 μmol m-2 s-1 PAR, adjustable between 5 and 50 μs. Multiple turnover flashes, maximal 30 000 μmol m-2 s-1 PAR, adjustable between 1 and 1000 ms | Far-red LED lamp: 720 nm. Chip-on-board (COB) LED array: 635 nm for continuous actinic illumination, maximum 4000 μmol m-2 s-1 PAR. Saturating single turnover flashes, maximal 200 000 μmol m-2 s-1 PAR, adjustable between 5 and 50 μs. Multiple turnover flashes, maximal 20 000 μmol m-2 s-1 PAR, adjustable between 1 and 1000 m |
| Actinic blue light | Using DUAL-DB: Blue (460 nm) LED lamp for continuous actinic illumination, maximum PAR 1100 μmol m-2 s-1. Chip-on-board LED array identical to that of DUAL-E measuring head
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Blue LED lamp: 460 nm for continuous actinic illumination, maximal 500 μmol m-2 s-1 PA |
| Signal detection | PIN photodiode with special pulse preamplifier for measuring P700 and fluorescence changes with maximal time resolution of 30 μs. Fluorescence is detected at wavelengths longer than 700 nm | PIN photodiode with special pulse preamplifier for measuring P700 and fluorescence changes with maximal time resolution of 10 μs |
| Communication | USB 1.1, 2.0 and 3.0 compatible | |
| User interface | Windows computer (Windows 10/11) with DualPAM software | |
Accessories for Modular Version
- DUAL-PAM Gas-Exchange Cuvette 3010-DUAL
- Linear Positioning System 3010-DUAL/B
- P515/535 Emitter Head DUAL-EP515
- P515/535 Detector Head DUAL-DP515
- 90 degree measuring head holder DUAL-H90
- Cuvette for Low-Drift Absorbance Measurement DUAL-K25
- Optical for Suspensions ED-101US/MD
- Temperature Control Block ED-101US/T
- Miniature Magnetic Stirrer PHYTO-MS
- Spherical Micro Quantum Sensor US-SQS/WB
- Emitter Head DUAL-ENADPH
- Detector Head DUAL-DNADPH
- Acridine Orange Emitter Head DUAL-EAO
- Acridine Orange Detector Head DUAL-DAO
Accessories for Fiber Version
- Miniature Magnetic Stirrer PHYTO-MS
- Suspension Cuvette KS-2500
- Magnetic Stirrer with Fiberoptics Holder MKS-2500
- Arabidopsis Leaf Holder DUAL-BA
- Dark Leaf Clip DLC-8
Support Documents
No resources found
Case Studies and Application Notes
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