The CI-301AD allows the user to adjust the CO2 and H2O concentrations of the leaf in the chamber and investigate the effects, ranging from 0-2000 ppm CO2 and 0-100% relative humidity.
Carbon dioxide concentration is a limiting factor for photosynthesis. If there is not enough CO2, then the light independent reaction of photosynthesis will not be able to take place as quickly. The direct effect is that less ribulose biphosphate will be made, which means that the whole photosynthesis reaction will be slowed down. The lack of carbon dioxide will cause a lack of products being made from the light independent stage to be reduced. During photosynthesis, water is necessary as a reactant.
Burner, David M. “Influence of alley crop environment on orchardgrass and tall fescue herbage.” Agronomy journal 95, no. 5 (2003): 1163-1171.
Swanborough, P. W., D. Doley, R. J. Keenan, and D. J. Yates. “Photosynthetic characteristics of Flindersia brayleyana and Castanospermum australe from tropical lowland and upland sites.” Tree physiology 18, no. 5 (1998): 341-347.