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Enabling better global research outcomes in soil, plant & environmental monitoring.

Ampha Z32 for Impedance Flow Cytometry

Amphasys' new-generation flow cytometer is a plug & play device designed to blend simplicity with flexibility. The absence of optical components reduces set-up times and maintenance to a minimum. There are no time-consuming sample preparation procedures and expensive fluorescent dyes. Latest generation digital signal processing electronics are specifically tailored to Amphasys' semi-disposable chips, such that best-of-class impedance analysis on single cells is achieved.

The small dimensions of Ampha Z32 and the robustness of the electrical measurement provide a portable solution for any kind of cell analysis, be it in your laboratory or in the field. Designed initially for research purposes, Ampha Z32 is now particularly suitable for routine applications, where detailed, high-quality cell analyses and ease-of-use are major requirements.

The Amphasys Ampha Z32 is ideal for field pollen viability measurement.

Controlled microfluidics

Interfacing microfluidic chips has always been a challenge, requiring high-end precision pumps and flow control devices for accurate and reproducible analyses. The robustness of the electrical measurement combined with the patented chip design and sophisticated data acquisition and processing algorithms enables the use of a rather simple peristaltic pump. Alongside, Ampha Z32 continuously monitors the amount of fluid that passes through the chip and thus permits accurate determination of cell concentrations from whole cultures or subpopulations. This simple fluidic system can be coupled to a bioreactor, for example, complementing Amphasys’ label-free approach and providing an elegant tool for in-line cell analysis applications.

AmphaSoft

The new AmphaSoft software intuitively combines instrument control and data acquisition, as well as realtime data display and offline data analysis. Therefore, no additional tools are needed for detailed data analysis. For routine analyses, Windows-based AmphaSoft provides a worklist facilitating repetitive analyses with changing parameters. Reports are generated in html- and csv- format, for stand-alone use or integration into proprietary templates, respectively.

Your application

Ampha Z32 has been used for countless research and industrial applications with any kind of cells, including bacteria and yeasts, animal and human cell lines as well as pollen and other plant cells. You can focus on the important tasks and run your routine analyses fully unattended using the Amphasys autosampler.
Typical analyses comprise, but are not limited to:

  • Cell size and morphology studies
  • Viability determination
  • Toxicology studies
  • Cell differentiation
  • Apoptosis
  • In-line cell monitoring

Amphasys’ new impedance flow cytometer Ampha Z32 provides a complementing technology to conventional fluorescence-based flow cytometers (FACS).

Ampha Z32 is the method of choice for detailed cell analysis demanding portable solutions, ease-of-use and quick results, as well as for all those applications in which optical systems fail because cell labelling is either not available, not applicable or disturbed by external factors.

Free to operate…

The mobility kit for Ampha Z32 enables you to perform your analyses wherever you like. A hard-top transport case protects the instrument from mechanical exposures, while the car adapter and/or the rechargeable Li-Ion battery permit you to work for the whole day independently from an electricity network. The robust device does not need any calibration, thus, just unpack the instrument, plug it to your car or the battery and start your analyses.

Impedance chip
Available sensing channel dimensions 15 x 15 μm, 30 x 30 μm, 50 x 50 μm, 80 x 80 μm, 120 x 120 μm or 250 x 250 μm
(depending on application requirements)
Impedance measurement
Frequency range 0.1 MHz – 30 MHz
Frequency selection Up to 4 different frequencies simultaneously
Analysis range
Sample volume 50 – 2000 μl (typically)
Concentration range 1 x 103 to 1 x 107 cells/ml
Particle size 1 – 150 μm
Fluidics
Sample flow rate 5 – 2500 μl/min (depending on channel dimension)
Pump Peristaltic pump with disposable pump head
Labware compatibility Standard 5 ml polystyrene round-bottom tubes
(Falcon® 352058 or Sarstedt 55.1579)
Operating system Laptop with Windows® 7 or 10
15” TFT LCD display
I5 or i7 QuadCore processor
128 GB SSD
Dimensions & weight
Dimensions (W x D x H) 255 x 275 x 353 mm
Weight 8.4 kg
Operating environment
Temperature 16° – 32°C
Humidity 10% – 90% relative non-condensing
Power 24VDC ± 10%, max. 3A, < 90 W
  • Z32 Autosampler
    Save time by automating sample loading for the Ampha Z32. With the Autosampler you can process up to 192 samples per run, giving you time back to focus on other important aspects of your project. The fully automated procedure includes sample loading, measuring and data recording.

Bokshi, A. I., Tan, D. K. Y., & Trethowan, R. M. (2019). A robust and rapid pollen viability test using impedance flow cytometry for high throughput screening of heat tolerant wheat (Triticum aestivum) germplasm. Proceedings of the 19th Australian Agronomy Conference. Presented at the Cells to Satellites 2019, Wagga Wagga, NSW, Australia. Retrieved from agronomyaustraliaproceedings.org/images/sampledata/2019/2019ASA_Bokshi_Anowarul_55.pdf

Crocetti, S., Beyer, C., Unternährer, S., Benavides Damm, T., Schade-Kampmann, G., Hebeisen, M., Di Berardino, M., Fröhlich, J. and Franco-Obregón, A. (2014), Impedance flow cytometry gauges proliferative capacity by detecting TRPC1 expression. Cytometry Part A, 85(6), 525–536. https://doi.org/10.1002/cyto.a.22461

Heidmann I., Di Berardino M. (2017) Impedance Flow Cytometry as a Tool to Analyze Microspore and Pollen Quality. In A. Schmidt (Ed.), Plant Germline Development: Methods and Protocols (pp. 339–354). https://doi.org/10.1007/978-1-4939-7286-9_25

Heidmann, I., Schade-Kampmann, G., Lambalk, J., Ottiger, M., Di Berardino, M. (2016) Impedance Flow Cytometry: A Novel Technique in Pollen Analysis. PLOS ONE, 11(11), e0165531. https://doi.org/10.1371/journal.pone.0165531

Ostermann, M., Birkeland, E., Xue, Y., Sauter, A., & Cimpan, M. R. (2017). Validation of an Impedance-Based Flow Cytometry Technique for High-Throughput Nanotoxicity Screening. ICN: International Conference on Nanoparticles, 11(3), 1744–1744. Retrieved from http://waset.org/abstracts/65613

Xu, J., Driedonks, N., Rutten, M. J. M., Vriezen, W. H., de Boer, G.-J., & Rieu, I. (2017). Mapping quantitative trait loci for heat tolerance of reproductive traits in tomato (Solanum lycopersicum). Molecular Breeding, 37(5), 58. https://doi.org/10.1007/s11032-017-0664-2