WATER-PAM
About the System
WATER-PAM-II
The WATER-PAM-II is a highly sensitive and compact chlorophyll fluorometer that measures chlorophyll a and the photosynthetic activity of samples with very low chlorophyll content. It features a portable cuvette system, is lightweight, and can easily be switched between red and blue light modes.
It can also be used as a stand-alone device for simple measurements, or connected to a computer to run a series of commands or scripts through WinControl-3 software to conduct analysis protocols such as induction and light curves.
The WATER-PAM-II features differential analysis of algal content, determining the composition of algal populations across three groups (green algae, cyanobacteria, and brown algae) based on F-spectrum variation.
WATER-PAM Fiber Version
The Fiber version is also available and enables users to examine photosynthetic layers on various submerged surfaces (periphyton, microphytobenthos), soil crusts, and to screen samples on microwell plates. Because the Fiber version is less sensitive than the WATER-PAM-II, users should maintain a chlorophyll concentration of approximately 100–500 µg chl a/L.
The WATER-PAM Fiber comes in two configurations:
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WATER-EDF1.5R: This configuration uses red measuring and actinic light and includes an additional blue LED, which selectively excites Photosystem I in cyanobacteria for F₀′ determination.
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WATER-EDF1.5B: This configuration uses blue LEDs instead of red and employs a far-red LED light source to excite Photosystem I in non-cyanobacterial photosynthetic organisms.
Included in the package:
WATER-PAM-II
- Optoelectronic unit
- AA Batteries
- Battery charger
- Mains power supply
- 1 quartz glass cuvette
- 5 standard glass cuvettes
- Cuvette holder
- Light Sensor
- USB cable
- Stylus
- Transport box
Fiber Version
- Emitter-Detector-Fiberoptics Unit (red) WATER-EDF1.5R
- Emitter-Detector-Fiberoptics Unit (blue) WATER-EDF1.5B
- Power Supply MINI-PAM-II/N
- Battery Charger 000190101101
- Universal Control Unit PAM-CONTROL
- Transport Box WATER-PAM/T
On this Page
Email: sales@ictinternational.com.au
Phone: 02 6772 6770 (between 9am to 5pm New South Wales time)
Schedule a meeting with our experts
Specifications:
WATER-PAM-II
| Parts | Specifications |
| Optoelectronic Unit WATER-PAM-II | |
| Measuring light | Blue (450 nm) and red (630 nm) LED, standard modulation frequencies 5 to 25 Hz, adjustable in increments of 5 Hz, and 100 Hz, measuring light PAR at standard settings = 0.05 µmol m-2 s-1. For differential algae analysis auxiliary LEDs 520 and 660 nm. |
| Actinic light | Blue (450 nm) and red (630) LED as for measuring light, maximum actinic PAR = 3000 µmol m-2 s-1, maximum PAR of saturation pulses = 6000 µmol m-2 s-1 adjustable in increments of 500 µmol m-2 s-1 expandable on request to 12000 µmol m-2 s-1 adjustable in increments of 1000 µmol m-2 s-1 |
| Far-red light | Peak emission at 730 nm |
| Signal detection | Hamamatsu photomultiplier protected by long-pass and a short-pass filters |
| Cuvette holder | for 15 mm Ø cuvette, Perspex inlet for overspill protection, POM cuvette cover 35 mm high x 40 mm Ø |
| Data memory | Flash memory, 8 MB, providing memory for more than 27,000 saturation pulse analyses |
| Ports | PAR sensor, Temp, AUX1, AUX2, SYNC, Stirrer, CTRL1, CTRL2, COMP, EXT DC and USB |
| Power supply | 8 AA (Mignon) rechargeable batteries (Eneloop 1.2 V/2 Ah), providing power for up to 1000 yield measurements; 8 spare batteries, automatic power/off, battery charger (100 to 240 V AC, 50-60 Hz, 0.35 A) for 1 to 8 AA NI-MH batteries, 12 V 5,5 A power supply MINI PAM-II/N |
Fiber Version
| Parts | Specifications | |
| Universal Control Unit PAM-CONTROL | ||
| Data memory | 128 kB CMOS RAM providing memory for 4000 data sets | |
| Display | 2 x 24-character alphanumerical LC-display with backlight | |
| Measured parameters | F0, FM, FM‘, F, FV/FM (max. Yield), ΔF/FM‘ (Yield), qP, qN, NPQ, PAR (using special micro quantum sensor), ETR (i.e. PAR x ΔF/FM‘) | |
| Power supply | Internal rechargeable battery 12 V/2 Ah, providing power for at least 10 000 Yield measurements, automatic power/off, battery charger MINI-PAM/L (100 to 240 V AC) | |
| Dimensions | 17.6 cm x 11.5 cm x 9.5 cm (L x W x H) | |
| Weight | 2 kg (incl. battery) | |
| Connectors | Actinic light
Drives up to three actinic LEDs AUX input Receives signals from temperature and light sensors AUX output Sends control signals for external LED drivers Charge Socket for battery charger Far-red light Up to three far red LEDs can be driven Measuring light Capacity to drive up to three LEDs both as measuring and actinic light Output Provides analog signal to chart recorder Photomultiplier Full control of photomultiplier PM-MF RS232 Data transfer to computer using WinControl V. 2 software
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| Â | Emitter-Detector-Fiberoptics Unit (red) WATER-EDF1.5R | Emitter-Detector-Fiberoptics Unit (blue) WATER-EDF1.5B |
| LED Array | Two red measuring light LEDs (620 nm) and two red actinic light LEDs (635 nm), all equipped with a filter blocking radiation > 700 nm. 1 blue LED (460 nm) for selective excitation of photosystem I in cyanobacteria. Note: the blue LED of the WATER-EDF1.5R is controlled by the Far-Red LED switch in the WinControl-3 software. Actinic intensity up to 2000 µmol quanta m‑2 s‑1 of photosynthetically active radiation (PAR). Saturation pulse intensity up to 4000 µmol quanta m‑2 s‑1. Dimensions: 11.5 cm x 9 cm x 5.5 cm (L x W x H) Weight: 550 g (incl. cable 0.6 m long) |
Two blue measuring light LEDs (460 nm) and two blue actinic light LEDs (460 nm). One far red LED (730 nm) for selective excitation of photosystem I in non-cyanobacterial photosynthetic organisms. Actinic intensity up to 2000 µmol quanta m‑2 s‑1 of photosynthetically active radiation (PAR). Saturation pulse intensity up to 4000 µmol quanta m‑2 s‑1. |
| Signal detection | Photomultiplier detector based on Photosensor Module H‑6779‑01 (Hamamatsu) with high red sensitivity; featuring pulse preamplifier and automatic overload switch-off; fluorescence detection at wavelengths > 710 nm | |
| Fiber Optics | Three 1.5 mm diameter plastic fiber optics (lengths 200 mm, 600 mm, and 1200 mm) for connection with the emitter-detector box are supplied. | |
Accessories
- Barcode Scanner BCS-9590
- Set of 5 standard glass cuvettes K5SET
- Flow-through cuvette WATER-II/FT
- Tripod Holder WATER-II/H
- Controllable Stirring Device WATER-II/S
- Quartz Glass Cuvette WATER-K
- Stirring Paddles WATER-R
Support Documents
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Case Studies and Application Notes
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