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PHYTO-PAM-II

Multiple Excitation Wavelength Phytoplankton & Photosynthesis Analyser
The PHYTO-PAM-II provides detailed differential analysis of photosynthetic performance across four algal groups using five measuring light wavelengths, six actinic light colours, and far-red (FR) illumination. The instrument detects fluorescence with extremely high sensitivity, down to concentrations of 0.1 µg Chl a per litre, which are typically found in natural surface waters and coastal seawater. In addition to analysing photosynthetic performance, the system offers a fast kinetics mode that assesses the wavelength-dependent absorption cross-section of PS II, σII(λ), by evaluating O–I₁ kinetics in single cultures.
PHYTO-PAM-II with • Multi-Color-Emitter Unit and Photomultiplier-Detector Unit

About the System

PHYTO-PAM-II Compact Version

The PHYTO-PAM-II Compact Version combines lightweight design with easy portability. Its splash-proof cast aluminium housing integrates the control unit along with all essential components of the emitter–detector system, including the optical unit, measuring and actinic LED array, photomultiplier detector, and cuvette retainer. This version can be equipped with the Flow-Through Cuvette PHYTO-II/FT. When paired with the 0–10 V Universal Pump Control PHYTO-II/FT/I, it allows users to operate common laboratory peristaltic pumps for software-controlled automated sampling.

PHYTO-PAM-II Modular Version

The PHYTO-PAM-II Modular Version offers greater flexibility than the Compact Version and allows users to customise the system based on their experimental setup. It is primarily designed for laboratory applications but can also operate in the field, as its power-and-control unit runs on a rechargeable battery. The Modular Version features exchangeable detector filters and two additional optical ports. It also provides options for software-triggered stirring and temperature control.

Included in the package:

Compact version

  • Power-and-control unit
  • PHYTO-II/FT Flow-Through Cuvette
  • Transport box

Modular Version

  • Stand with Base Plate ST-101
  • Power-and-Control-Unit PHYTO-II-C
  • Multi-Color-Emitter Unit PHYTO-II-E
  • Photomultiplier-Detector Unit – PHYTO-II-D
  • Battery Charger MINI-PAM/L
  • Temperature Control Unit US-T
  • Optical Unit for Suspensions ED-101US/MD
  • Transport Box

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Specifications:

Compact Version

Parts Specifications
Control Unit
Chip-on-board multi-wavelength measuring light LED emitter 440, 480, 540, 590, and 625 nm for pulse-modulated measuring light; 2 intensity settings; 8 settings of pulse frequency and 3 settings of auto MF-high pulse frequency
Chip-on-board multi-wavelength actinic LED array 440, 480, 540, 590, 625 and 420-640 nm (white) for continuous actinic illumination, up to 1500 µmol m-2 s-1 PAR; fast kinetic flashes up to 7000 µmol m-2 s-1 PAR; saturation pulse up to 5000 µmol m-2 s-1 PAR,
Far-Red LED peak wavelength 725 nm
Signal detection Photomultiplier detector
Standard detector filter long-pass filter > 650 nm
Sockets charge socket for Battery Charger MINI-PAM/L, input socket for US-SQS/WB Spherical Micro Quantum Sensor, USB socket
Ports PAR sensor, Temp, AUX1, AUX2, SYNC, Stirrer, CTRL1, CTRL2, COMP, EXT DC and USB
Power supply Rechargeable sealed lead-acid battery 12 V/2 Ah; Battery Charger MINI-PAM/L (100 to 240 V AC)
PHYTO-II/FT Flow-Through Cuvette Cuvette holder from black polyoxymethylene plastic (POM) with in-/out water tubing connectors (4 mm inner Ø and M 5 screw), to be mounted with two knurled-head screws to PHYTO-PAM-II compact version instruments. Delivery package includes a POM mounting device for light sensor US-SQS/WB.

Modular Version

Parts Specifications
Power-and-Control-Unit PHYTO-II-C
Sockets CHARGE socket for Battery Charger MINI-PAM/L, AUX socket for US-SQS/WB Spherical Micro Quantum Sensor, USB socket, socket for Miniature Magnetic Stirrer PHYTO-MS, DETECTOR socket for connecting PHYTO-II-D Photomultiplier-Detector Unit, LED ARRAY and FLUO ML sockets for connecting PHYTO-II-E Multi-Color-Emitter Unit
User interface Windows computer with PhytoWin-3 software
Power supply Rechargeable sealed lead-acid battery 12 V/2 Ah; Battery Charger MINI-PAM/L (100 to 240 V AC)
Multi-Color-Emitter Unit PHYTO-II-E
Chip-on-board multi-wavelength measuring light LED emitter 40, 480, 540, 590 and 625 nm for pulse-modulated measuring light; 2 intensity settings; 8 settings of pulse frequency and 3 settings of Auto MF-high pulse frequency
Chip-on-board multi-wavelength actinic LED array 440, 480, 540, 590, 625 and 420-640 nm (white) for continuous actinic illumination, up to 3000 µmol m-2 s-1 PAR; actinic pulses for Fast Kinetics, up to 11000 µmol m-2 s-1 PAR; saturation pulses up to 12000 µmol m-2 s-1 PAR
Photomultiplier-Detector Unit – PHYTO-II-D
Signal detection Photomultiplier detector based on Photosensor Module H-10720 (Hamamatsu)
Standard detector filter long-pass filter > 650 nm

 

Accessories

  • Miniature Magnetic Stirrer PHYTO-MS
  • Micro Quantum Sensor US-SQS/WB

Support Documents

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