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Physiology and Genotyping of Adaptive Oil Palm Progenies under Unwatered Stress

Abstract

Increasing of dry areas for Oil Palm plantation due to climate change led the breeders to develop drought stress, tolerant palm. Characterization of genetic materials for their physiological and genetic properties is required. Two distinct progenies derived from Tanzanian origin used for more adaptive to unwatered stress, and Nigerian origin used for more sensitive, were subjected for analysis. Physiological related variables were observed for sap flow, water potential, soil water content, greenhouse temperature and relative humidity, stomatal density, stomatal pore width, and proline content. The Tanzanian origin has more stable in sap flow, longer water potential detected, more adaptive in stomatal closure, and a lower in proline content than Nigerian origins. The genome comparison of both materials was performed by double digest Restriction Associated DNA and captured 299 294 SNPs. It found 9.3 % of those SNPs that were having a different allele with a minor frequency between progenies. And, 0.7 % from filtered SNPs were located and distributed in 167 genes. Two SNP markers were located in aquaporin NIP1-1 gene that related to water translocation between cells. One SNP marker was located in glutamate receptor 2.7-like gene that related to glutamate release and proline biosynthesis. The remaining SNPs will be in further analyzed.

Keywords: Aquaporin, glutamate, proline, sap flow, SNP, stomata, water potential

 

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ICT Instruments Used in Research Paper

1. SFM = SFM1 Sap Flow Meter

2. PM Psychrometer = PSY / PSY1 Stem Psychrometer

3. SMM = SMM1 Soil Moisture Meter

 

Yono, D., Purwanti, E., Sahara, A., Nugroho, Y. A., Tanjung, Z. A., Aditama, R., … & Liwang, T. (2019, June). Physiology and genotyping of adaptive and sensitive oil palm progenies under unwatered stress condition. In IOP Conference Series: Earth and Environmental Science (Vol. 293, No. 1, p. 012012). IOP Publishing. doi:10.1088/1755-1315/293/1/012012